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ppm1a overexpression vector  (OriGene)


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    OriGene ppm1a overexpression vector
    Ppm1a Overexpression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 97/100, based on 1852 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppm1a overexpression vector/product/OriGene
    Average 97 stars, based on 1852 article reviews
    ppm1a overexpression vector - by Bioz Stars, 2026-02
    97/100 stars

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    <t>PPM1A</t> was a downstream target of miR-140-3p. A The predicted binding sequence of miR-140-3p and PPM1A was shown. B Relative luciferase activity was determined by dual-luciferase reporter assay in RA-FLSs co-transfected with PPM1A 3′UTR WT and PPM1A 3′UTR MUT and miR-NC or miR-140-3p. C , D The mRNA and protein expression of PPM1A was examined in synovial tissues from RA patients ( n = 22) and normal patients ( n = 22) by qRT-PCR and western blot assays, respectively. E , F PPM1A mRNA and protein levels were determined in RA-FLSs and control H-FLSs by qRT-PCR and western blot assays, respectively. G The enrichment of miR-140-3p was examined via qRT-PCR in RA-FLSs with transfection of miR-NC or miR-140-3p. H , I The mRNA and protein levels of PPM1A in RA-FLSs with transfection of miR-NC or miR-140-3p were tested by qRT-PCR and western blot assays, respectively. J , K PPM1A mRNA and protein levels were detected in RA-FLSs with transfection of si-NC, si-circMAPK9#2, si-circMAPK9#2 + anti-miR-NC, or si-circMAPK9#2 + anti-miR-140-3p by qRT-PCR and western blot assays, respectively. * P < 0.05
    Ppm1a Overexpression Vector (Ppm1a, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppm1a overexpression vector (ppm1a/product/Ribobio co
    Average 90 stars, based on 1 article reviews
    ppm1a overexpression vector (ppm1a - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    ppm1a overexpression vector  (OriGene)
    97
    OriGene ppm1a overexpression vector
    <t>PPM1A</t> was a downstream target of miR-140-3p. A The predicted binding sequence of miR-140-3p and PPM1A was shown. B Relative luciferase activity was determined by dual-luciferase reporter assay in RA-FLSs co-transfected with PPM1A 3′UTR WT and PPM1A 3′UTR MUT and miR-NC or miR-140-3p. C , D The mRNA and protein expression of PPM1A was examined in synovial tissues from RA patients ( n = 22) and normal patients ( n = 22) by qRT-PCR and western blot assays, respectively. E , F PPM1A mRNA and protein levels were determined in RA-FLSs and control H-FLSs by qRT-PCR and western blot assays, respectively. G The enrichment of miR-140-3p was examined via qRT-PCR in RA-FLSs with transfection of miR-NC or miR-140-3p. H , I The mRNA and protein levels of PPM1A in RA-FLSs with transfection of miR-NC or miR-140-3p were tested by qRT-PCR and western blot assays, respectively. J , K PPM1A mRNA and protein levels were detected in RA-FLSs with transfection of si-NC, si-circMAPK9#2, si-circMAPK9#2 + anti-miR-NC, or si-circMAPK9#2 + anti-miR-140-3p by qRT-PCR and western blot assays, respectively. * P < 0.05
    Ppm1a Overexpression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppm1a overexpression vector/product/OriGene
    Average 97 stars, based on 1 article reviews
    ppm1a overexpression vector - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

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    PPM1A was a downstream target of miR-140-3p. A The predicted binding sequence of miR-140-3p and PPM1A was shown. B Relative luciferase activity was determined by dual-luciferase reporter assay in RA-FLSs co-transfected with PPM1A 3′UTR WT and PPM1A 3′UTR MUT and miR-NC or miR-140-3p. C , D The mRNA and protein expression of PPM1A was examined in synovial tissues from RA patients ( n = 22) and normal patients ( n = 22) by qRT-PCR and western blot assays, respectively. E , F PPM1A mRNA and protein levels were determined in RA-FLSs and control H-FLSs by qRT-PCR and western blot assays, respectively. G The enrichment of miR-140-3p was examined via qRT-PCR in RA-FLSs with transfection of miR-NC or miR-140-3p. H , I The mRNA and protein levels of PPM1A in RA-FLSs with transfection of miR-NC or miR-140-3p were tested by qRT-PCR and western blot assays, respectively. J , K PPM1A mRNA and protein levels were detected in RA-FLSs with transfection of si-NC, si-circMAPK9#2, si-circMAPK9#2 + anti-miR-NC, or si-circMAPK9#2 + anti-miR-140-3p by qRT-PCR and western blot assays, respectively. * P < 0.05

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: CircMAPK9 promotes the progression of fibroblast-like synoviocytes in rheumatoid arthritis via the miR-140-3p/PPM1A axis

    doi: 10.1186/s13018-021-02550-y

    Figure Lengend Snippet: PPM1A was a downstream target of miR-140-3p. A The predicted binding sequence of miR-140-3p and PPM1A was shown. B Relative luciferase activity was determined by dual-luciferase reporter assay in RA-FLSs co-transfected with PPM1A 3′UTR WT and PPM1A 3′UTR MUT and miR-NC or miR-140-3p. C , D The mRNA and protein expression of PPM1A was examined in synovial tissues from RA patients ( n = 22) and normal patients ( n = 22) by qRT-PCR and western blot assays, respectively. E , F PPM1A mRNA and protein levels were determined in RA-FLSs and control H-FLSs by qRT-PCR and western blot assays, respectively. G The enrichment of miR-140-3p was examined via qRT-PCR in RA-FLSs with transfection of miR-NC or miR-140-3p. H , I The mRNA and protein levels of PPM1A in RA-FLSs with transfection of miR-NC or miR-140-3p were tested by qRT-PCR and western blot assays, respectively. J , K PPM1A mRNA and protein levels were detected in RA-FLSs with transfection of si-NC, si-circMAPK9#2, si-circMAPK9#2 + anti-miR-NC, or si-circMAPK9#2 + anti-miR-140-3p by qRT-PCR and western blot assays, respectively. * P < 0.05

    Article Snippet: PPM1A overexpression vector (PPM1A) based on the pcDNA3.1 vector and the empty pcDNA3.1 vector (vector) were also acquired from Ribobio.

    Techniques: Binding Assay, Sequencing, Luciferase, Activity Assay, Reporter Assay, Transfection, Expressing, Quantitative RT-PCR, Western Blot

    The influence of miR-140-3p and PPM1A on cell malignant progression and inflammatory response in RA-FLSs. RA-FLSs were transfected with miR-NC, miR-140-3p, miR-140-3p + vector, or miR-140-3p + PPM1A. A Western blot assay was performed to detect the protein expression of PPM1A. Cell proliferation ( B ), apoptosis ( C ) and cycle distribution ( D ), migration ( E ), and invasion ( F ) were measured by MTT assay, flow cytometry analysis, or transwell assay, respectively. G Western blot was carried out to examine the protein expression of Bax, Bcl-2, MMP2, and MMP9. H – J The levels of TNF-α, IL-1β, and IL-6 were analyzed by ELISA. * P < 0.05

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: CircMAPK9 promotes the progression of fibroblast-like synoviocytes in rheumatoid arthritis via the miR-140-3p/PPM1A axis

    doi: 10.1186/s13018-021-02550-y

    Figure Lengend Snippet: The influence of miR-140-3p and PPM1A on cell malignant progression and inflammatory response in RA-FLSs. RA-FLSs were transfected with miR-NC, miR-140-3p, miR-140-3p + vector, or miR-140-3p + PPM1A. A Western blot assay was performed to detect the protein expression of PPM1A. Cell proliferation ( B ), apoptosis ( C ) and cycle distribution ( D ), migration ( E ), and invasion ( F ) were measured by MTT assay, flow cytometry analysis, or transwell assay, respectively. G Western blot was carried out to examine the protein expression of Bax, Bcl-2, MMP2, and MMP9. H – J The levels of TNF-α, IL-1β, and IL-6 were analyzed by ELISA. * P < 0.05

    Article Snippet: PPM1A overexpression vector (PPM1A) based on the pcDNA3.1 vector and the empty pcDNA3.1 vector (vector) were also acquired from Ribobio.

    Techniques: Transfection, Plasmid Preparation, Western Blot, Expressing, Migration, MTT Assay, Flow Cytometry, Transwell Assay, Enzyme-linked Immunosorbent Assay